2. Signaling Pathways
  3. Cell Cycle/DNA Damage
  4. DNA/RNA Synthesis

DNA/RNA Synthesis

DNA/RNA Synthesis

Related Products

PDF 1.61 MB

RNA synthesis, which is also called DNA transcription, is a highly selective process. Transcription by RNA polymerase II extends beyond RNA synthesis, towards a more active role in mRNA maturation, surveillance and export to the cytoplasm.

Single-strand breaks are repaired by DNA ligase using the complementary strand of the double helix as a template, with DNA ligase creating the final phosphodiester bond to fully repair the DNA.DNA ligases discriminate against substrates containing RNA strands or mismatched base pairs at positions near the ends of the nickedDNA. Bleomycin (BLM) exerts its genotoxicity by generating free radicals, whichattack C-4′ in the deoxyribose backbone of DNA, leading to opening of the ribose ring and strand breakage; it is an S-independentradiomimetic agent that causes double-strand breaks in DNA.

First strand cDNA is synthesized using random hexamer primers and M-MuLV Reverse Transcriptase (RNase H). Second strand cDNA synthesis is subsequently performed using DNA Polymerase I and RNase H. The remaining overhangs are converted into blunt ends using exonuclease/polymerase activity. After adenylation of the 3′ ends of DNA fragments, NEBNext Adaptor with hairpin loop structure is ligated to prepare the samples for hybridization. Cell cycle and DNA replication are the top two pathways regulated by BET bromodomain inhibition. Cycloheximide blocks the translation of mRNA to protein.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-200129
    PCNA-IN-1
    		Inhibitor 98.30%
    PCNA-IN-1 (Compound 11) is a PCNA/PIP-box interaction inhibitor, with IC50 of >50 μM. PCNA-IN-1 can be used in research of cancer.
    PCNA-IN-1
  • HY-W048491
    2′-O-(2-Methoxyethyl)adenosine
    		≥99.0%
    2′-O-(2-Methoxyethyl)adenosine is a compound can be used in the synthesis of oligonucleotides.
    2′-O-(2-Methoxyethyl)adenosine
  • HY-W105447
    3'-TBDMS-ibu-rG Phosphoramidite
    		≥98.0%
    3'-TBDMS-ibu-rG Phosphoramidite is a phosphorite monomer that can be used in the synthesis of oligonucleotides.
    3'-TBDMS-ibu-rG Phosphoramidite
  • HY-W013049S1
    Docosanoic acid-d43
    		Inhibitor 99.29%
    Docosanoic acid-d43 (Behenic acid-d43) is the deuterium labeled Docosanoic acid (HY-W013049). Docosanoic acid (Behenic acid) is a long-chain saturated fatty acid. Docosanoic acid inhibits the double-stranded DNA (dsDNA) binding activity of p53 DNA binding domain, with a Kd of 12 nM. Docosanoic acid has low bioavailability and can increase cholesterol in humans.
    Docosanoic acid-d<sub>43</sub>
  • HY-B1777AS
    Spermine-d8 tetrahydrochloride
    Spermine-d8 (tetrahydrochloride) is the deuterium labeled Spermine tetrahydrochloride. Spermine tetrahydrochloride is a polyamine nitric oxide donor that can provide nitric oxide to platelets and inhibit platelet activation to a certain extent concentration-dependently. Spermine tetrahydrochloride occurs in mammalian tissues, plants, bacteria, ribosomes and bacteriophage. Spermine tetrahydrochloride inhibits primary human embryo lung fibroblasts in vitro. Spermine is a natural antioxidant and anti-inflammatory agent. Spermine is known to inhibit some bacterial cultures, especially strains of Staphylococcus aureus. Spermine induces neurotoxicity in the striarum dose-dependently. Spermine can reversibly inhibits DNA synthetic response, mixed lymphocyte response and the induction of cytolytic lymphocyte response in primary cultures of murine spleen cells.
    Spermine-d<sub>8</sub> tetrahydrochloride
  • HY-Y1055R
    Guanine (Standard)
    		Chemical
    Guanine (Standard) is the analytical standard of Guanine. This product is intended for research and analytical applications. Guanine is one of the fundamental components of nucleic acids (DNA and RNA). Guanine is a purine derivative, consisting of a fused pyrimidine-imidazole ring system with conjugated double bonds. Guanine has the potential to serve as a large-capacity N pool.
    Guanine (Standard)
  • HY-W008821
    1,7-Heptanediamine
    		Inhibitor 98.54%
    1,7-Heptanediamine (C7) is a deoxyhypusine synthase inhibitor with IC50 value of 4.5 μM.
    1,7-Heptanediamine
  • HY-B0268
    Enoxacin
    		Inhibitor 98.67%
    Enoxacin (AT 2266), a fluoroquinolone, interferes with DNA replication and inhibits bacterial DNA gyrase (IC50=126 µg/ml) and topoisomerase IV (IC50=26.5 µg/ml). Enoxacin is a miRNA processing activator and enhances siRNA-mediated mRNA degradation and promotes the biogenesis of endogenous miRNAs. Enoxacin has potent activities against gram-positive and -negative bacteria. Enoxacin is a cancer-specific growth inhibitor that acts by enhancing TAR RNA-binding protein 2 (TRBP)-mediated microRNA processing.
    Enoxacin
  • HY-W077279
    5'-O-DMT-N4-Ac-dC
    5'-O-DMT-N4-Ac-dC (N4-Acetyl-2'-deoxy-5'-O-DMT-cytidine, compound 7), a deoxynucleoside, can be used to synthesize of dodecyl phosphoramidite which is the raw material for dod‐DNA (amphiphilic DNA containing an internal hydrophobic region consisting of dodecyl phosphotriester linkages) synthesis.
    5'-O-DMT-N4-Ac-dC
  • HY-Y1055A
    Guanine hydrochloride
    		98.78%
    Guanine hydrochloride is one of the fundamental components of nucleic acids (DNA and RNA). It is a purine derivative composed of a fused pyrimidine-imidazole ring system with conjugated double bonds. Guanine hydrochloride has the potential to serve as a large-capacity nitrogen reservoir.
    Guanine hydrochloride
  • HY-W039722
    5-Fluorocytidine
    		Inhibitor 99.91%
    5-Fluorocytidine is a member of cytidines, inhibits maturation of the 45S ribosomal RNA precursor.
    5-Fluorocytidine
  • HY-W012311
    3′-Deoxythymidine
    		Inhibitor ≥98.0%
    3′-Deoxythymidine (2′,3′-Dideoxythymidine), a nucleoside analog, inhibits eukaryotic cellular and viral DNA polymerases, and inhibits retrovirus infection in some cells.
    3′-Deoxythymidine
  • HY-18572S2
    2,4-D-d5
    		99.39%
    2,4-D-d5 is the deuterium labeled 2,4-D. 2,4-D (2,4-Dichlorophenoxyacetic acid) is a selective systemic herbicide for the control of broad-leaved weeds. 2,4-D acts as a plant hormone, causing uncontrolled growth in the meristematic tissues. 2,4-D inhibits DNA and protein synthesis and thereby prevents normal plant growth and development.
    2,4-D-d<sub>5</sub>
  • HY-W116007
    Octamethylcyclotetrasiloxane
    		Inhibitor 99.85%
    Octamethylcyclotetrasiloxane promotes the anchorage-independent growth of MCF-10A and MCF-10F cells. Octamethylcyclotetrasiloxane induces DNA damage, inhibits the expression of DNA-repairing protein BRCA1 under long-term and high-concentration exposure. Octamethylcyclotetrasiloxane exhibits intrinsic estrogenic activity.
    Octamethylcyclotetrasiloxane
  • HY-152246
    ZIM
    		99.88%
    ZIM, a norbornene derived from 4-Aminoantipyrine, is a potent inducer of DNA damage, causing genomic and chromosomal damage as well as inducing cell death and activating phagocytosis. ZIM has chemotherapeutic potential for use in cancer research.
    ZIM
  • HY-138614
    5'-O-DMT-2'-O-TBDMS-Ac-rC
    		99.80%
    5'-O-DMT-2'-O-TBDMS-Ac-rC is a modified nucleoside and can be used to synthesize DNA or RNA.
    5'-O-DMT-2'-O-TBDMS-Ac-rC
  • HY-161431
    RTx-152
    		99.71%
    RTx-152 traps Polθ on DNA and is an allosteric Polθ-pol inhibitor (IC50: 6.2 nM). RTx-152 selectively kills HR-deficient cancer cells, and suppresses PARP inhibitor resistance in multiple genetic backgrounds, including homologous recombination (HR)-proficient cells. RTx-152 selectively kills BRCA2-null cells.
    RTx-152
  • HY-138598
    5'-O-TBDMS-dG
    		98.83%
    5'-O-TBDMS-dG is a modified nucleoside. 5'-O-DMT-2'-O-TBDMS-rI can be used in the synthesis of deoxyribonucleic acid or nucleic acid.
    5'-O-TBDMS-dG
  • HY-107372S4
    Uridine triphosphate-15N2 dilithium
    		≥98.0%
    Uridine triphosphate-15N2 (UTP-15N2 dilithium; Uridine 5'-triphosphate-15N2) dilithium is 15N labeled Uridine triphosphate (HY-107372). Uridine triphosphate (UTP;Uridine 5'-triphosphate) is a nucleotide that regulates the functions of the pancreas in endocrine and exocrine secretion, proliferation, channels, transporters, and intracellular signaling under normal and disease states.
    Uridine triphosphate-<sup>15</sup>N<sub>2</sub> dilithium
  • HY-119840
    Anthragallol
    		99.55%
    Anthragallol can insert base pairs of DNA. Anthragallol exhibits cytotoxicity by binding to DNA.
    Anthragallol
Cat. No. Product Name / Synonyms Application Reactivity